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COMPOSITION CONTAINING DNA CODING FOR A RIBOZYME AND AN OLIGONUCLEOTIDE SUBSTRATE, AND METHOD FOR MEASURING TRANSCRIPTION RATES
COMPOSITION CONTAINING DNA CODING FOR A RIBOZYME AND AN OLIGONUCLEOTIDE SUBSTRATE, AND METHOD FOR MEASURING TRANSCRIPTION RATES
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机译:含有核糖核酸和寡核苷酸底物的DNA编码的组合物,以及测定转录速度的方法
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摘要
Compositions (reporter systems) are described, the a a ribozyme - dna coding sequence, and an oligonucleotide substrate, the of the of the dna - sequence transcribed ribozyme is cleaved. In a preferred embodiment, the oligonucleotide substrate with a fluorophoric group (reporter group) and a fluorescence - be erased group (quencher group), after cleavage with the ribozyme the erasing of the fluorescence of the fluorophore by means of the fluorescence - be erased group is prevented, i.e, a fluorescence signal is generated. Furthermore, a process for the measurement of transcription rates are described, for example, for the determination of inhibitors of the transcription or activators.
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