首页> 外国专利> COMPOSITION CONTAINING DNA CODING FOR A RIBOZYME AND AN OLIGONUCLEOTIDE SUBSTRATE, AND METHOD FOR MEASURING TRANSCRIPTION RATES

COMPOSITION CONTAINING DNA CODING FOR A RIBOZYME AND AN OLIGONUCLEOTIDE SUBSTRATE, AND METHOD FOR MEASURING TRANSCRIPTION RATES

机译:含有核糖核酸和寡核苷酸底物的DNA编码的组合物,以及测定转录速度的方法

摘要

Compositions (reporter systems) are described, the a a ribozyme - dna coding sequence, and an oligonucleotide substrate, the of the of the dna - sequence transcribed ribozyme is cleaved. In a preferred embodiment, the oligonucleotide substrate with a fluorophoric group (reporter group) and a fluorescence - be erased group (quencher group), after cleavage with the ribozyme the erasing of the fluorescence of the fluorophore by means of the fluorescence - be erased group is prevented, i.e, a fluorescence signal is generated. Furthermore, a process for the measurement of transcription rates are described, for example, for the determination of inhibitors of the transcription or activators.
机译:描述了组合物(报道系统),核酶-dna编码序列,和寡核苷酸底物,切割了转录的dna-序列的核酶的序列。在一个优选的实施方案中,具有荧光基团(报告基团)和荧光-被擦除基团(猝灭剂基团)的寡核苷酸底物,在用核酶切割后,通过荧光-被擦除基团擦除荧光团的荧光。防止荧光,即产生荧光信号。此外,描述了用于测量转录速率的方法,例如用于确定转录抑制剂或激活剂的方法。

著录项

  • 公开/公告号EP1064405A1

    专利类型

  • 公开/公告日2001-01-03

    原文格式PDF

  • 申请/专利权人 JENNE ANDREAS;FAMULOK MICHAEL;

    申请/专利号EP19990915650

  • 发明设计人 FAMULOK MICHAEL;JENNE ANDREAS;

    申请日1999-03-17

  • 分类号C12Q1/68;C12Q1/44;C12N9/00;

  • 国家 EP

  • 入库时间 2022-08-22 01:16:22

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