METHOD AND DEVICE FOR THE RAPID LIQUID CHROMATOGRAPHIC SEPARATION OF SUBSTANCE MIXTURES AND FOR THE IDENTIFICATION OF SUBSTANCES

摘要

Rapid liquid chromatographic substance separation and identification comprises software controlled two-stage separation using a second parallel fine separation, identification and isolation stage. Rapid liquid chromatographic separation and identification of substances is performed by software controlled two-stage separation comprising a first stage of initially separating a substance mixture and a second stage using two or more separation lines for parallel fine separation of the separated fractions and parallel identification and isolation of the finely separated fractions. An Independent claim is also included for an apparatus for carrying out the above process, comprising a separation column (10) with several parallel liquid chromatographic separation lines. Each line comprises a combination of separation column batteries (11-13) with collector column batteries (7-9), detector units (14) and fraction collector units (15.1,15.2,15.3). A pump unit (2), with three pumps (2.1, 2.2, 2.3) used for transporting the mobile phase, is connected to the separation column (10) and to the separation lines and software operated multi-way valves are positioned between the individual functional units. Preferred Features: The first separation stage involves initial separation of successive substances and the second stage involves successive and/or parallel fine separation of substances. A detector (14.1,14.2,14.3) is provided after each separation stage and a multi-way valve (3.5,3.6,3.7) is provided before each separation line, further separation columns being positioned downstream of the separation lines.