- Recombinant vector harboring murine GM-CSF and recombinant Aspergilus niger expressing the vector

摘要

PURPOSE: Provided are a recombinant vector including murine granulocyte macrophage colony stimulating factor and recombinant Aspergillus niger expressing the factor. Transformed Aspergillus niger mass-produces murine granulocyte macrophage colony stimulating factor to overcome side effects caused by over dose of the factor and can be applied to clinic. CONSTITUTION: A recombinant vector, optionally being pANGM-CSF plasmid is composed of a promotor of glycelaldehyde 3-phosphoric acid dehydrogenase, a terminator of glycelaldehyde 3-phosphoric acid dehydrogenase and a murine granulocyte macrophage colony stimulating factor. A recombinant host cell can be selected from Aspergillus niger, Fusarium oxysporum, Cryphonectria parasitica, Aspergillus nidulans, and Aspergillus awamori. In particular, Aspergillus niger (KCTC 0661BP) is favorable. The optimum conditions for the expression of a recombinant protein are as follows: pH 5.0-6.0 and the temperature of 30-37 deg.C.