METHOD FOR PRODUCING A BACULOVIRUS VECTOR CAPABLE OF PRODUCING A SELECTED NON-FUSED RECOMBINANT PROTEIN OR CAPABLE OF BEING UTILIZED AS A TRANSFER VECTOR;A RECOMBINANT BACULOVIRUS EXPRESSION VECTOR CAPABLE OF EXPRESSING UNDER THE CONTROL OF A BACULOVIRUS POLYHEDRIN PROMOTER A SELECTED NON-FUSED PROTEIN IN A HOST INSECT CELL;A RECOMBINANT TRANSFER VECTOR UTILIZING A BACULOVIRUS POLYHEDRIN PROMOTER AND FLANKING SEQUENCES FOR INTRODUCING A SELECTED GENE INTO A BACULOVIRUS GENOME,OR FOR INSERTION OF A SELECTED GENE FOR A GENETIC MANIPULATION OF A BACULOVIRUS;AND A METHOD FOR THE SYNTHESIS OF A SELECTED NON-FUSED PROTEIN IN A HOST INSECT CELL USING THE EXPRESSION VECTOR

摘要

A method for producing a recombinant baculovirus expression vector, capable of expressing a selected gene in a host insect cell is disclosed. The method involves cleaving baculovirus DNA to produce a DNA fragment comprising a polyhedrin gene or portion thereof, including a polyhedrin promoter. A recombinant transfer vector is prepared by inserting said DNA fragment into a cloning vehicle and thereafter inserting a selected gene into the thus modified cloning vehicle such that it is under the transcriptional control of the polyhedrin promoter. The recombinant transfer fector is contacted with a baculovirus DNA so as to effect recombination and incorporation of the selected gene into the baculovirus genome. The resultant recombinant baculovirus is then used to infect susceptible insects or cultured insect cells and the protein product from the incorporated selected gene is produced from the infection.