Method for large-scale culture of the cell which produces rearrangement hitoerisuropoiechin

摘要

(57) Abstract Generally, it rearranges this invention, in the culture nutrient medium which includes the insulin, regards the method for large-scale culture of the rearrangement mammal cell production for hitoerisuropoiechin (EPO). In addition this invention, following the method of producing EPO, and to that, you call also EPO which is produced. This invention states the method for large-scale culture of the appropriate rearrangement cell for the industrial production of EPO. Mass EPO to which the method of being stated in this invention includes the careless protein of density low in the culture nutrient medium and does not expect is produced. This condition raises the EPO refinement process after, and causes high protein recovery factor. The yield whose EPO which can by the method of long-cherished desire invention is high is achieved by the addition of the insulin to the culture nutrient medium.