HUMAN MANGANESE SUPEROXIDE DISMUTASE CDNA, ITS EXPRESSION IN BACTERIA AND METHOD OF RECOVERING ENZYMATICALLY ACTIVE HUMAN MANGANESE SUPEROXIDE DISMUTASE

摘要

A double-stranded cDNA molecule which includes DNAencoding human manganese super oxide dismutase has beencreated. The sequence of one strand of a double-stranded DNA molecule which encodes human manganesesuperoxide dismutase has been discovered. Such mole-cules may be introduced in procaryotic, e.g., bacteri-al, or eukaryotic, e.g. , yeast or mammalian, cells andthe resulting cells cultured or grown under suitableconditions sa as to produce human manganese superoxidedismutase or analogs thereof which may then be recov-ered. Human MnSOD or analogs thereof may be used tocatalyze the reduction of superoxide radicals, reducereperfusion injury, prolong the survival time of iso-lated organs, or treat inflammations.The invention also concerns a method of producing enzy-matically active human manganese superoxide dismutaseor an analog thereof in a bacterial cell which containsand is capable of expressing a DNA sequence encodingthe superoxide dismutase by maintaining the bacterialcell under suitable conditions and in a suitable pro-duction medium. The production medium is supplementedwith an amount of Mn++ so that the concentration ofMn++ in the medium is greater than about 2 ppm.This invention also concerns a method of recoveringpurified enzymatically active manganese super oxidedismutase from bacterial cells.