DETERMINATION OF NUCLEIC ACID BY PCR, MEASUREMENT OF NUMBER OF MICROBIAL CELLS, GENES, OR GENE-COPIES BY PCR, AND MEASURING-KIT EMPLOYED FOR THE SAME

摘要

A PCR amplification product is detected, inquantitative determination of nucleic acid andmeasurement of the number of bacterial cells orspecific genes, by addition of a dye compound whichdoes not fluoresce in the free state but fluoresces inthe bonded state to a double-stranded nucleic acid.