The invention features methods of determining whether a nucleotide sequence encodes an MDR efflux pump, methods for deleting a desired region of DNA in a bacterial cell, methods for determining whether a test substance inhibits the growth or metabolism of cells of a strain of iE. faecalis/i bacteria having a disruptive mutation in a gene encoding a MDR efflux pump, methods for determining whether a test substance includes a compound that blocks efflux of an antibacterial agent from a cell, and methods for identifying an inhibitor of an MDR pump.
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