Processes for the systematic eradication of pathogenic agents carried by animals and compositions used in these processes

摘要

Systematic eradication of pathogenic agents from animals before transport or installation into housing comprises: (i) spraying walls and ceiling of buildings uniformly with an aqueous solution containing a bacterial complex containing at least two non pathogenic Bacillus (i.e. B.subtilis, B.amyloliquefaciens and B.megaterium), each at a concentration of 102 to 5 x 104 cfu/ml, at least two non pathogenic Lactobacillus (i.e. L.paracesei and L.rhamnosus) and at least one non pathogenic Pediococcus, each at a concentration of 104 to 5 x 108 (especially 5 x 107) cfu/ml, and associated with a water-soluble nutritive diluent, where the spraying comprises: (a) spraying at the start of preparation of the buildings for reception of the animals; (b) spraying 12-48 hours after (a); and (c) optionally spraying about 7 days later, this spraying programme being repeated each time for a new flock of animals; (ii) evenly spreading the floor of the building with a composition in solid powder or granular form comprising: (a') bacterial complex comprising at least one B. subtilis and at least one other non pathogenic Bacillus (i.e. B.amyloliquefaciens, B.megaterium, B.licheniformis and B.circulans), each at a concentration of 103 to 106 cfu/g, at least one non pathogenic Lactobacillus (i.e. L.paracasei, L.rhamnosus, L.fermentum and L.acidophilus) and at least one non pathogenic Pediococcus, each at a concentration of 103 to 108 cfu/g; (b') at least one nutritive diluent, (c') at least one diluent with electrostatic properties; and (d') at least one support for fixing the microparticles, the ratio bacterial complex and diluents : support being (2:98)-(15:85) (especially 5:95) % w/w, where the spreading comprises :(a'') spreading onto the floors of the buildings before installing the litter or other covering, (b'') installing the litter, if used, followed by spreading over the litter and (c'') after installing the animals, spreading the agent over the whole of the floor and litter or covering once a week; and (iii) if necessary, spraying material to be used in the building with a solution comprising a bacterial complex containing at least two non pathogenic Bacillus (i.e. B.subtilis, B.amyloliquefaciens and B.megaterium), each at a concentration of 104 to 5 x 108 cfu/ml, at least two non pathogenic Lactobacillus (i.e. L. paracasei and L.rhamnosus) and at least one non pathogenic Pediococcus, each at a concentration of 104 to 5 x 108 (especially 106 to 5 x 107) cfu/ml, in association with water-soluble nutritive diluent (i.e. sources of aminoacids e.g. yeast extract) at 0.01-5% and sugars; and (iv) after installing the animals, systematically adding probiotic products to the animal feed.