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NEW XYLOGLUCAN OLIGOSACCHARIDE-DEGRADING ENZYME, GENE ENCODING THE SAME, AND METHOD FOR PRODUCING THE ENZYME

机译:一种新的氧化木葡糖寡糖降解酶,编码该酶的基因以及生产该酶的方法

摘要

PROBLEM TO BE SOLVED: To provide a new xyloglucan oligosaccharide-degrading enzyme having a degradation style different from those of known enzymes.;SOLUTION: This method for producing the enzyme comprises collecting from a microorganism belonging to the genus Geotrichum the enzyme specifically degrading the second β-glucoside bond from the reducing terminal among β-glucoside bonds constituting the main chain of the xyloglucan oligosaccharide, clarifying the amino acid sequence and gene base sequence of the enzyme, and then highly purely producing a polypeptide having the enzyme activity at a low cost by a genetic engineering means.;COPYRIGHT: (C)2003,JPO
机译:解决的问题:提供一种新的木葡聚糖低聚糖降解酶,其降解方式与已知酶不同;解决方案:该酶的生产方法包括从属于土t属的微生物中收集专门降解第二种酶的酶。构成木葡聚糖寡糖的主链的β-葡萄糖苷键中的还原端的β-葡萄糖苷键,澄清了酶的氨基酸序列和基因碱基序列,然后高纯度地产生了具有酶活性的多肽。通过基因工程手段降低成本。;版权所有:(C)2003,日本特许厅

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