CLONING OF GENES ENCODING FOR CELLULOLYTIC ENZYMES FROM METAGENOME IN GOAT'S RUMEN

摘要

The increasing fuel demands has caused in rise in price of crude oil and so the need for alternative source of bioenergy. Among the potential alternative bioenergy resources, cellulosic biomass is considered as a sustainable raw material for biofuel production. The cellulosic bioconversion into fermentable sugar is a complex process and requires the synergistic action of endoglucanases, exoglucanases and ss-glucosidases. Those enzymes are hosted in the microbial genomes in the rumen of herbivores. To be able to tap into those resources is expected to have an significant impact on cellulosic biofuel production, but a majority of those microbial species are not accessible using laboratory culture techniques. This study was performed to develop molecular approaches for using these genome resources.