首页> 外国专利> CULTURE MEDIUM FOR SEPARATING SOY SAUCE LACTIC BACTERIUM HAVING LOW TURBIDITY, METHOD FOR SEPARATING SOY SAUCE LACTIC BACTERIUM HAVING LOW TURBIDITY BY USING THE MEDIUM, AND METHOD FOR PRODUCING SOY SAUCE HAVING HIGH CLARITY BY USING THE LACTIC BACTERIUM

CULTURE MEDIUM FOR SEPARATING SOY SAUCE LACTIC BACTERIUM HAVING LOW TURBIDITY, METHOD FOR SEPARATING SOY SAUCE LACTIC BACTERIUM HAVING LOW TURBIDITY BY USING THE MEDIUM, AND METHOD FOR PRODUCING SOY SAUCE HAVING HIGH CLARITY BY USING THE LACTIC BACTERIUM

机译:分离度低的酱油酸乳酸菌的培养剂,用培养基分离度低的酱油酸乳酸菌的方法和分离度高的大豆酸化的酱油的方法

摘要

PROBLEM TO BE SOLVED: To provide a culture medium capable of readily providing a low-turbidity soy sauce Lactobacillus strain having low survival ability under low pH environment, having high hydrophilic degree of cell surfaces and property which is readily decomposable by an enzymatic group derived from Aspergillus oryjae and to provide a method for simply separating the low-turbidity soy sauce Lactobacillus strain by using the culture medium and to readily obtain soy sauce having high clarity by using the lactic bacterium.;SOLUTION: A bacterium to be examined is inoculated into an agar culture medium containing ≥0.12% (W/V) neomycin (potency: 650 U/mg) and the bacterium is cultured and the grown bacterial strain is separated or the bacterium to be examined is inoculated into an agar nutrient culture medium containing 0.2-0.25% (W/V) cobalt chloride and not containing common salt and cultured and the grown bacterial strain is separated or 0.2-1.0% (W/V) aspartic acid, arginine, tyrosine, histidine, or phenylalanine is added to a basic culture medium to sterilize the basic culture medium and the bacterium to be examined is inoculated into the culture medium and cultured and whether a decomposition product exists or not is observed and a bacterial strain not decomposing any amino acid is separated.;COPYRIGHT: (C)2003,JPO
机译:解决的问题:提供一种培养基,该培养基能够容易地提供低浊度的酱油乳杆菌菌株,该菌株在低pH值环境下的存活能力低,具有高亲水性的细胞表面和性质,并且容易被衍生自酶的基团分解。通过使用培养基简单地分离低浊度酱油乳杆菌菌株并通过使用乳酸菌容易地获得具有高澄清度的酱油的方法;解决方案:将待检查的细菌接种到大肠杆菌中。含有0.12%(W ​​/ V)新霉素的琼脂培养基(效价:650 U / mg)进行细菌培养并分离生长的细菌菌株或将待检查的细菌接种到含有0.2的琼脂营养培养基中-0.25%(W / V)的氯化钴,不含食盐,经培养可分离生长的细菌菌株,或分离0.2-1.0%(W / V)的天冬氨酸,精氨酸,酪氨酸向碱性培养基中加入肌苷,组氨酸或苯丙氨酸以对碱性培养基进行灭菌,然后将待检查的细菌接种到该培养基中并进行培养,观察是否存在分解产物,并且细菌菌株不会分解分离氨基酸;版权所有:(C)2003,日本特许厅

著录项

  • 公开/公告号JP2003079363A

    专利类型

  • 公开/公告日2003-03-18

    原文格式PDF

  • 申请/专利权人 KIKKOMAN CORP;

    申请/专利号JP20010272018

  • 发明设计人 MABUCHI KIYOTO;

    申请日2001-09-07

  • 分类号C12N1/20;A23L1/238;C12N1/00;

  • 国家 JP

  • 入库时间 2022-08-22 00:16:36

相似文献

  • 专利
  • 外文文献
  • 中文文献
获取专利

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号