Stepwise solid-phase synthesis of peptide-oligonucleotide conjugates and supports therefor

摘要

A support for peptide-oligonucleotide conjugate synthesis includes an article of manufacture according to the formula: chemistry chemdraw filechemistry mol file;wherein: (a) substitutent A includes a polymeric base material and/or a silica base material; (b) one of substituents B and C includes an NHFmoc-containing group and/or a peptide-containing group; (c) one of substituents B and C includes an NHBoc-containing group, an O-DMTr-containing group, and/or an oligonucleotide-containing group; and (d) each X independently represents ═O, S, and/or NH. Suitable supports include those according to the formula: chemistry chemdraw filechemistry mol file;wherein substituents A, B, and C have the meanings described above. When used to synthesize a peptide-oligonucleotide conjugate, the resulting conjugate may have the formula: chemistry chemdraw filechemistry mol file;wherein substituent B includes a peptide and substituent C includes an oligonucleotide.