Devices and methods are provided to Pierce the skin and accessing and Collecting Physiological Sample inside. The devices include at least one trajectory of Fluid, wherein at least a substantial part of the distal end of the at least one Fluid Path is open to the outside environment.One or more devices can be integrated to form a Strand of analysis to determine the concentration of at least one analyte in the sample. Also provided are methods of using the Devices of the case.The devices and methods are particularly suitable for Collecting Physiological Sample and determine glucose concentrations inside and, more particularly, Concentrations of blood glucose, blood fractions or Interstitial Fluid. Also provided are teams that include Devices for use in the implementation of the methods of the case.
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