PREPARATION OF RED BLOOD CELLS WITH A MODIFIED LEVEL OF BLOOD GROUP ANTIGEN EXPRESSION AND THEIR USE IN THE QUALITY CONTROL OF BLOOD TYPING REAGENTS

摘要

A method for the preparation of red blood cells expressing reduced levels ofblood group antigens using at least oneimmunodominant sugar modifying enzyme, for example alpha-N-acetylgalactosaminidase or alpha-galactosidase. The red bloodcells produced preferably express a level of antigen substantially equivalentto the clinically significant threshold for the antigen.Red blood cells produced according to said method are used for the qualitycontrol of blood typing reagents and the calibration oftesting systems to give accurate and standardized determinations of bloodgroup types.