首页> 外文学位 >Identifying and Characterizing Red Blood Cell Microvesiculation, Phosphatidylserine and CD47 Expression As a Predictor of Red Blood Cell In Vitro Quality Following Hypothermic Storage.
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Identifying and Characterizing Red Blood Cell Microvesiculation, Phosphatidylserine and CD47 Expression As a Predictor of Red Blood Cell In Vitro Quality Following Hypothermic Storage.

机译:鉴定和表征红细胞微囊化,磷脂酰丝氨酸和CD47表达是低温保存后红细胞体外质量的预测指标。

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摘要

Proper preservation of the quality of stored red blood cells (RBC) is fundamental for successful blood transfusion. RBC microparticles (RMPs) have been shown to be harmful effectors of inflammation and coagulation posttransfusion, as well as in vitro indicators of RBC function and integrity throughout ex vivo storage. The main objectives of this thesis were to develop a flow cytometric analysis method to identify, characterize and quantify RMPs during hypothermic storage (HS) along with examining the effects of biochemical rejuvenation on RBC microvesiculation and membrane composition. This thesis demonstrated that HS conditions induce RBC microvesiculation and changes in CD47 and PS. This study also showed evidence that ATP restoration following the rejuvenation had no significant effect on RMPs generation or PS and CD47 expression throughout HS. The work presented in this thesis has furthered the field of the biopreservation by outlining techniques to improve blood transfusion.
机译:正确保存储存的红细胞(RBC)的质量是成功输血的基础。 RBC微粒(RMP)已被证明是输血后炎症和凝血的有害效应物,并且是整个离体储存过程中RBC功能和完整性的体外指标。本论文的主要目的是开发一种流式细胞仪分析方法,以鉴定,表征和定量低温保存(HS)过程中的RMP,同时研究生化复兴对RBC微囊泡和膜组成的影响。本论文证明HS条件诱导RBC微囊泡形成并改变CD47和PS。这项研究还表明,复兴后ATP的恢复对整个HS的RMP生成或PS和CD47表达没有明显影响。本文提出的工作通过概述改善输血的技术进一步拓展了生物保存领域。

著录项

  • 作者

    Almizraq, Ruqayyah Jassim.;

  • 作者单位

    University of Alberta (Canada).;

  • 授予单位 University of Alberta (Canada).;
  • 学科 Biology Cell.;Health Sciences Pathology.
  • 学位 M.S.
  • 年度 2013
  • 页码 167 p.
  • 总页数 167
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 老年病学;
  • 关键词

  • 入库时间 2022-08-17 11:41:37

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