METHOD FOR PRODUCING erythrocytic diagnosticums to detect antibodies to parainfluenza-3 cattle in the indirect agglutination

摘要

FIELD: medicine. SUBSTANCE: method involves carrying out hemagglutinins destruction after concentrating and purifying viral proteins, by applying 2- 3-fold freezing-defrosting until viral proteins solution is produced that does not agglutinate sheep erythrocytes. Sensitization is carried out at 50-56 C during 1 h 20 min at Ph equal to 6.0-6.6 when stirring the mixture every 20-30 min. The so produced diagnosticum corresponds to a titer of positive serum from 1:128 to 1:65536. EFFECT: accelerated reaction time; high reliability of diagnosis.