This indirect extraction of the dna of non-culturable and dna can be obtained by said other

摘要

The invention concerns a method for indirectly extracting DNA target with a size greater than 300 kB from non-cultivable organisms contained in an environmental sample which consists in: grinding said sample in liquid medium; recuperating the resulting ground material; separating the organisms from the rest of the ground material by sedimentation on a cushion with density higher than 1; recuperating the organisms isolated at the surface of the cushion and incorporating it in an agarose block; extracting the DNA by lysis in the agarose block; placing the block in an agarose gel which is subjected to at least pulsed field electrophoresis; finally, recuperating the extracted DNA strands.