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Luciferase detection reagent kits and luciferase detection method using the kit
Luciferase detection reagent kits and luciferase detection method using the kit
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机译:萤光素酶检测试剂盒及使用该试剂盒的萤光素酶检测方法
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摘要
The present invention provides a detection method and a method of manufacturing a detection kit, both characterized by use of an organic sulfur reagent, and which are effective at low concentration of the reagent, are inexpensive, and have reduced unpleasent odor. Provided is a reagent kit for detecting a Coleoptera luciferase, comprising an organic sulfur reagent having the atomic sequence of sulfur-carbon-sulfur in its chemical structure, a luciferin, adenosine triphosphate and a magnesium ion. Also provide is a method for detecting a Coleoptera luciferase, comprising step 1 of mixing an aqueous solution, containing an organic sulfur reagent having the atomic sequence of sulfur-carbon-sulfur in its chemical structure, a luciferin, adenosine triphosphate and a magnesium ion, with a sample containing a Coleoptera luciferase, to give a mixed solution; and step 2 of measuring the light emitted in the mixed solution.
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