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NUCLEOTIDE SEQUENCES HAVING ACTIVITY OF CONTROLLING TRANSLATION EFFICIENCY AND UTILIZATION THEREOF
NUCLEOTIDE SEQUENCES HAVING ACTIVITY OF CONTROLLING TRANSLATION EFFICIENCY AND UTILIZATION THEREOF
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机译:具有控制翻译效率和利用其活性的核苷酸序列
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摘要
The present invention provides a method for preparing a polynucleotide comprising a nucleotide sequence having an activity of regulating the translation efficiency of a template in a protein synthesis system, characterized by: (a) supplying one or more types of template comprising any nucleotide sequence to a protein synthesis reaction system; (b) recovering a polyribosomal fraction from the reaction solution after the reaction; and (c) collecting the polynucleotide comprising the nucleotide sequences in the template contained in the polyribosomal fraction. Also provided are a novel polynucleotide having an activity of regulating the translation efficiency produced by this method, and a protein synthesis method using a template comprising this polynucleotide.
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