NUCLEOTIDE SEQUENCES HAVING ACTIVITY OF CONTROLLING TRANSLATION EFFICIENCY AND UTILIZATION THEREOF

摘要

A method of preparing a polynucleotide containing a nucleotide sequence which has an activity of controlling the translation efficiency of a template in a protein synthesis system, characterized by comprising (a) applying a template containing one or more arbitrary nucleotide sequences to a protein synthesis reaction system, (b) after reacting, recovering a polyribosome fraction from the liquid reaction mixture, and (c) collecting a polynucleotide containing the nucleotide sequence in the template from the polyribosome fraction; novel polynucleotides having an activity of controlling translation efficiency which are obtained by the above method; a method of synthesizing a protein with the use of a template containing such a polynucleotide; and so on.