ISOTHERMAL POLYMERASE AMPLIFICATION (Polymerase isothermal amplification)

摘要

the ipa is Isothermal polymerase Amplification (u0438u0437u043eu0442u0435u0440u043cu0430u043bu044cu043du0430u00a0 u043fu043eu043bu0438u043cu0435u0440u0430u0437u043du0430u00a0 u0430u043cu043fu043bu0438u0444u0438u043au0430u0446u0438u00a0) - u0440u0435u0430u043au0446u0438u00a0, u043eu0441u043du043eu0432u0430u043du043du0430u00a0 the characteristic dna u043fu043eu043bu0438u043cu0435u0440u0430u0437u044b i (e).coli) catalyse the synthesis of dna on the nucleic acid in the previously synthesised circuits using 4 u0434u0435u0437u043eu043au0441u0438u0440u0438u0431u043eu043eu043bu0438u0433u043eu043du0443u043au043bu0435u043eu0442u0438u0434u043du044bu0445 u043fu0440u0430u0439u043cu0435u0440u043e in consistently u0438u043du0438u0446u0438u0438u0440u0443u044eu0449u0438u0445 reaction polymerization, which was then u0443u0434u0430u043bu00a0u044eu0442u0441u00a0 of matrices in a u0441u0442u0443u043fu0435u043du0447u0430u0442u043eu0433u043e u0443u0434u0430u043bu0435u043du0438u00a0 u043cu043eu043du043eu043du0443u043au043bu0435u043eu0442u0438u0434u043eu0432 with 5 / the end of the u0434u0432u0443u0445u0446u0435u043fu043eu0447 u0435u0447u043du043eu0439 dna u043eu0441u0432u043eu0431u043eu0436u0434u0430u00a0 spot annealing u0434u043bu00a0 u043fu0440u0430u0439u043cu0435u0440u043eu0432,but the 5 / 3 / u044du043au0437u043eu043du0443u043au043bu0435u0430u0437u043du0430u00a0 activity of this enzyme u0431u043bu043eu043au0438u0440u0443u0435u0442u0441u00a0 on u0441u0438u043du0442u0435u0437u0438u0440u0443u0435u043cu044bu0435 by u043fu0440u0438u043cu0435u043du0435u043du0438u00a0 u0442u0438u043eu043fu0440u043eu0438u0437u0432u043eu0434u043du044bu0445 u0434u0435u0437u043eu043au0441u0438u043du0443u043au043bu0435u043eu0437u0438u0434u0442u0440u0438u0444u043eu0441u0444u0430u0442u043eu0432 chain dna.