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METHOD FOR DETECTING BIFIDOBACTERIUM LONGUM BB536

机译:检测双歧杆菌BB536的方法

摘要

PROBLEM TO BE SOLVED: To provide a PCR primer which is specific to Bifidobacterium longum BB536, does not exhibit a cross reaction not only with other Bifidobacterium genus bacteria but also with the different strains of Bifidobacterium longum, and can quickly and simply detect the Bifidobacterium longum BB536, and to provide a detection method using the same.;SOLUTION: This PCR primer for detecting the Bifidobacterium longum BB536 comprises a pair of an oligonucleotide having a specific base sequence and an oligonucleotide having a specified base sequence. The method for detecting the Bifidobacterium longum BB536 comprises performing a PCR using the PCR primer and using the chromosomal DNA of a test bacterium as a template and then determining the presence or absence of an amplified product.;COPYRIGHT: (C)2006,JPO&NCIPI
机译:解决的问题:提供一种特异于长双歧杆菌BB536的PCR引物,该引物不仅与其他双歧杆菌属细菌没有交叉反应,而且与长双歧杆菌的不同菌株也没有交叉反应,并且可以快速,简单地检测到长双歧杆菌解决方案:该PCR引物用于检测长双歧杆菌BB536,包括一对具有特定碱基序列的寡核苷酸和具有特定碱基序列的寡核苷酸。检测长双歧杆菌BB536的方法包括使用PCR引物并以测试细菌的染色体DNA为模板进行PCR,然后确定是否存在扩增产物。版权所有:(C)2006,JPO&NCIPI

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