Using a method for selecting high-expressing cell lines producing and intron fusion constructs

摘要

Methods for producing selection methods DNA constructs, high-expressing host cells, a protein of interest at high rates, the invention relates to eukaryotic cell production method with multiple copies of the sequences encoding the protein of interest. In one method, stable clones that can be produced at a high level of product of interest are generated from one step direct selection at after transfection.