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Detection reagent for shiga toxin family gene of entero-hemorrhagic escherichia coli

机译：肠出血性大肠杆菌志贺毒素家族基因检测试剂

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摘要

The present invention provides a combination of oligonucleotides preferable for composing a rapid and specific gene testing reagent for Shiga toxin family gene type 2 (stx) of entero-hemorrhagic Escherichia coli (EHEC). More specifically, the present invention provides a method for detecting stx2 RNA of EHEC by specifically amplifying only stx2 RNA using a primer having a sequence homologous or complementary to a base sequence specific for stx2 gene of EHEC and located at sites free of alterations between genotypes, and an oligonucleotide that binds to a specific site of stx2 RNA.

机译：本发明提供了优选用于构成肠出血性大肠杆菌（IHEC）的志贺毒素家族2型（stx）的快速和特异性基因测试试剂的寡核苷酸组合。更具体地，本发明提供了一种通过使用具有与EHEC的stx2基因特异性的碱基序列同源或互补的序列并且位于没有基因型之间改变的位点的引物仅特异性地扩增stx2 RNA来检测EHEC的stx2 RNA的方法，以及与stx2 RNA特定位点结合的寡核苷酸。

著录项

公开/公告号US2006115823A1

专利类型
公开/公告日2006-06-01

原文格式PDF
申请/专利权人 NORIYOSHI MASUDA;KIYOSHI YASUKAWA;RYUICHI HORIE;
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申请/专利号US20050048794
发明设计人 NORIYOSHI MASUDA;KIYOSHI YASUKAWA;RYUICHI HORIE;
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申请日2005-02-03
分类号C12Q1/68;C12P19/34;
国家 US
入库时间 2022-08-21 21:45:22

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