SITE-SPECIFIC SERINE RECOMBINASES AND METHODS OF THEIR USE

摘要

The present invention provides a method for obtaining site-specificrecombination in a eukaryotic cell, the method comprising providing aeukaryotic cell that comprises a first recombination attachment site and asecond recombination attachment site; contacting the first and secondrecombination attachment sites with a prokaryotic recombinase polypeptide,resulting in recombination between the recombination attachment sites, whereinthe recombinase polypeptide can mediate recombination between the first andsecond recombination attachment sites, the first recombination attachment siteis a phage genomic recombination attachment site (attP) or a bacterial genomicrecombination attachment site (attB), the second recombination site is attB orattP, and the recombinase is selected from the group consisting of a Listeriamonocytogenes phage recombinase, a Streptococcus pyogenes phage recombinase, aBacillus subtilis phage recombinase, a Mycobacterium tuberculosis phagerecombinase and a Mycobacterium smegmatis phage recombinase, provided thatwhen the first recombination attachment site is attB, the second recombinationattachment site is attP and when the first recombination attachment site isattP, the second recombination attachment site is attB. The invention alsodescribes compositions, vectors, and methods of use thereof, for thegeneration of transgenic cells, tissues, plants, and animals. Thecompositions, vectors and methods of the present invention are also useful ingene therapy applications.