首页> 外国专利> NUCLEIC ACID AMPLIFICATION PRIMER FOR DETECTING RBCS-1A GENE AND METHOD OF QUANTIFYING NUCLEIC ACID BY USING THE GENE AS INTERNAL STANDARD

NUCLEIC ACID AMPLIFICATION PRIMER FOR DETECTING RBCS-1A GENE AND METHOD OF QUANTIFYING NUCLEIC ACID BY USING THE GENE AS INTERNAL STANDARD

机译:用于检测RBCS-1A基因的核酸扩增底物和利用该基因作为内部标准物定量核酸的方法

摘要

A primer for amplifying a nucleic acid characterized by being hybridizable with a target nucleic acid sequence containing at least one member selected from the group consisting of ribulose bisphosphate carboxylase small chain 1A: RBCS-1A) gene, mRNA of this gene, cDNA of this gene and partial sequences thereof and having a first sequence hybridizable with a part of the target nucleic acid sequence as described above in the 3’-terminal side and a second sequence hybridizable with a part of a complementary chain of the target nucleic acid sequence as described above in the 5’-terminal side; and a method of quantifying a nucleic acid by using at least one member selected from among the above-described gene, mRNA of the gene, cDNA of the gene and partial sequences thereof as an internal standard.
机译:用于扩增核酸的引物,其特征在于可与包含选自核糖二磷酸羧化酶小链1A:RBCS-1A)基因,该基因的mRNA,该基因的cDNA中的至少一个的靶核酸序列杂交。及其部分序列,并具有可在3'-末端侧与上述靶核酸序列的一部分杂交的第一序列和与上述靶核酸序列的互补链的一部分杂交的第二序列在5'端侧;以及通过使用选自上述基因,基因的mRNA,基因的cDNA及其部分序列中的至少一种作为内标来定量核酸的方法。

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