METHOD FOR THE IDENTIFICATION OF ENZYMES HAVING DESIRED PROPERTIES BY IMMOBILISATION OF THE REACTION PRODUCT ON THE SURFACE OF ENZYME-PRESENTING ORGANISMS

摘要

The invention relates to a method for the identification of enzymes with a desired activity, by random generation of a large collection of enzyme variants, the synthesis of said variants in host organisms, the presentation thereof on the surface of the organisms and the isolation of enzyme variants with the desired properties, by the detection of the covalent deposition of the reaction product on the surface of the host organism.