A TRANSGENIC PLATYCODON GRANDIFLORM RESISTANT TO A HERBICIDE

摘要

The present invention relates to a method for producing a herbicide resistant transgenic herbicide resistance gene by introducing in Bellflower Bellflower and its relates to a transformant obtained by the method bellflower . ; in the present invention, through a selection process from Agrobacterium tumefaciens and the inoculated medium with addition of antibiotics and containing the PAT gene using the cotyledon fragments of bellflower transformants were obtained , it was confirmed the insertion of the PAT, NPT and GUS gene by the PCR analysis , DNA of the PAT gene by RT-PCR analysis confirmed that the transcription in RNA. In addition, it was confirmed that the culture bottle switched Bellflower traits within the PPT (phosphinothricin) resistance .