A transgenic Platycodon grandiflorm resistant to a herbicide

摘要

The present invention is a method for producing a herbicide resistant transgenic Bellflower in Bellflower by introducing a herbicide tolerance gene and its relates to a transformant obtained by the method bellflower . ; in the present invention, through a selection process from Agrobacterium tumefaciens and the inoculated medium with addition of antibiotics and containing the PAT gene with fragments of the cotyledons bellflower transformants were obtained , were confirmed the insertion of the PAT, NPT and the GUS gene by the PCR analysis , DNA of the PAT gene by RT-PCR analysis confirmed that the transfer to RNA. In addition, it was confirmed that the culture bottle switched Bellflower traits within the PPT (phosphinothricin) resistance .