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RTX and related genes of Vibrio vulnificus responsible for the contact-cytotoxicity and lethality to animals
RTX and related genes of Vibrio vulnificus responsible for the contact-cytotoxicity and lethality to animals
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机译:RTX和创伤弧菌相关基因负责动物的接触细胞毒性和致死性
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摘要
PURPOSE: RTX toxin and related genes of Vibrio vulnificus responsible for the contact-cytotoxicity and lethality to animals are provided, thereby producing mutant strain with attenuated cytotoxicity by deletion of the cytotoxicity related genes. CONSTITUTION: The mutant strain with attenuated cytotoxicity is produced by deletion of one or more genes in RTX operon of Vibrio vulnificus which are selected from rtxB2 gene of SEQ ID NO:5, rtxD gene of SEQ ID NO:7, rtxB1 gene of SEQ ID NO:3, ORF(open reading frame) between the rtxB1 gene and rtxC protein of SEQ ID NO:11; rtxC gene of SEQ ID NO:9 and rtxA gene of SEQ ID NO:1. An alive vaccine against Vibrio vulnificus is produced by using the RTX toxin production deficient mutant strain.
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机译:目的:提供负责对动物的接触细胞毒性和致死性的创伤弧菌的RTX毒素和相关基因,从而通过缺失细胞毒性相关基因而产生具有减弱的细胞毒性的突变菌株。组成:具有减弱的细胞毒性的突变菌株是通过缺失创伤弧菌的RTX操纵子中的一个或多个基因产生的,所述基因选自SEQ ID NO:5的rtxB2基因,SEQ ID NO:7的rtxD基因,SEQ ID NO:7的rtxB1基因NO:3,rtxB1基因与SEQ ID NO:11的rtxC蛋白之间的ORF(开放阅读框); SEQ ID NO:9的rtxC基因和SEQ ID NO:1的rtxA基因。通过使用RTX毒素产生缺陷突变株,可以生产出针对创伤弧菌的活疫苗。
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