A culture-free method for detection of Vibrio vulnificus from coastal seawater based on loop-mediated isothermal amplification targeting vcgC gene

摘要

Vibrio vulnificus is an opportunistic pathogen widely distributed in estuarine and coastal seawaters.In this study,a culture-free method was developed to rapid detection of V.vulnificus in all seasons,based on loop-mediated isothermal amplification(LAMP) targeting virulent-correlated gene(vcg).The new assay method allows differentiation between the virulent and non-virulent strain of V.vulnificus accurately.This method also allows effective detection of the pathogeny in winter when the bacterium lives in the viable but non-culturable(VBNC) state.A total of 30 costal seawater samples collected in all seasons were used for the evaluation of this method.The results show that the method is sensitive,accurate and convenient.