The present invention includes the steps of fixing the hydrogel or PEG- hydrogel copolymer having a functional group on a substrate; Coupling to the hydrogel or hydrogel by PEG- copolymer is added to a mixed solution of a fixed substrate comprising a salt and a nucleic acid sample wherein said nucleic acid hydrogel or PEG- hydrogel copolymer; The step of washing the nucleic acids bound hydrogel or PEG- hydrogel copolymer; And eluting it using a solvent for the hydrogel or hydrogel PEG- separation and purification of nucleic acids using immobilized copolymer, comprising the step of eluting the bound nucleic acid. In accordance with the invention to remove the DNA from the substrate to immobilize the hydrogel or PEG- hydrogel copolymer on a substrate, if the tablet was bound and eluted the DNA with no addition of a separate chemical, effects on subsequent processes, such as PCR can be minimized, and can adjust the amount and strength of binding of the DNA to be combined according to the PEG concentration adjustment, the patterning is possible because of the hydrogel type compound is present on the substrate.
展开▼
