Model system for testing of pharmaceutical preparations for effectiveness in the case of inflammatory processes in the nervous system

摘要

The invention relates to a method for testing of active ingredients in pharmaceutical preparations on their neuroprotective or inflammation module atoroidal effect. By means of the combination of cytokines, which act on the microglia and cause, inter alia, their proliferation, with an ignition of the soluble constituents ends substance (endotoxin lipopolysaccharide as imflammogen) can be an excessive inflammatory response in organotypical cultures of brain be initiated, which to the degeneration of brain tissue and thus also leads to destruction of neurons. As our invention, this reaction, which in the case of all chronic and acute neuropathies a disastrous role in organotypical cultures, which retain the complete complexity of the brain tissue, close to the suspected pathological processes in the patient''s brain be recapitulates. The inventive model system enables the search for pharmaceutical preparations with either a neuroprotective or inflammation modulatory action, which with good predictability both in the animal model and in the patient their healing effect.