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A method for improving the stability and the widening of the ph - region of the g / 11 xylanases family
A method for improving the stability and the widening of the ph - region of the g / 11 xylanases family
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机译:一种提高g / 11木聚糖酶家族ph区域稳定性和拓宽的方法。
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摘要
The present invention relates to protein engineering, and concerns especially family G/11 xylanases, and genes encoding said enzymes. In specific, the invention concerns Trichoderma reesei XYNII gene, which codes for endo-1,4-β-xylanase (EC 3.2.1.8). The invention describes how site-directed mutagenesis can be used to improve the properties of an enzyme to match the industrial conditions where it is used. Protein engineering can be used to improve thermoactivity and thermostability of xylanases, as well as to broaden their pH range.
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