Xylanases from families 10 and 11 from an extreme thermophile. bleaching activity on kraft pulp and evolutionary improvement of xynb

摘要

Genes for xylanases form two different glycosyl hydrolase families from the bacterim Dictyoglmus thermophilum Rt46B. 1 have been siolated and over-expressed in bacterial and fungal hosts. XynB has shown greater promise than XynA as a beeach-boosting agent for both pine and ecualyt kraft pulps in ECF and TCF sequences. Becuse of the limits of natural biodiversity, futhrer development of XynB for increased pH range is being explored by enzyme evolution techniques which combine in vitro mutagenesis and recombination. Simulations using in sikico population genertics are also being implemented to explore the available sequence space.