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Assay method for detecting infectious prion protein in a sample of a mammalian subject, kit for use in a assay method, and use of a binding antibody
Assay method for detecting infectious prion protein in a sample of a mammalian subject, kit for use in a assay method, and use of a binding antibody
TEST METHOD FOR DETECTION OF INFECTIOUS PRI PROTEIN IN SAMPLE OF A MAMMALIAN, KIT FOR USE IN A TEST METHOD, AND USE OF A CONNECTION ANTIBODY The present invention provides a test method for detecting prion infectious protein. in a sample from a mammalian subject, said method comprising: obtaining a sample containing the prion protein from said individual; contacting said sample with an agent, which serves to digest the noninfectious prion protein and partially digesting the infected prion protein to produce a prion protein polypeptide residue: contacting the digested sample with an antibody capable of bind to a polypeptide having the amino acid sequence Vc (Gly-Gly-Gly-Trp) -Gly-Gly-Gly-Gly-R1-1 -R2-2-His-R3- Asn-Lys-Pro-R-4-Lys-Pro-Lys-Thr-R-5-R-6-Lys (His-R-7-Ala-Gly) (Vc) and detect conjugates of said antibody and said prion protein polypeptide residue; wherein the detection of said conjugates comprises chemical, biological or biochemical amplification of detectable species and detection of the amplified species.
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