A GENE DELIVERING VECTOR COMPRISING THE GENE ENCODING SOLUBLE AND SECRETABLE HOMOTRIMER FORMING RECOMBINANT HUMAN TRAIL

摘要

A gene-delivering vector comprising a gene encoding a recombinant human TRAIL(TNF(tumor necrosis factor)-related apoptosis-inducing ligand) having ability of forming soluble and secretable homotrimer is provided to inhibit growth of cancer cells, induce apoptosis of cancer cells, and improve safety of use by eliminating infectious properties from the vector through removing E1 and E3 genes associated with adenovirus replication. A shuttle vector pAdloxTRAIL for producing replication-incompetent adenovirus comprises an expression cassette containing a secretion signal sequence of SEQ ID NO:3, a trimer-forming domain of SEQ ID NO:4 and TRAIL gene of SEQ ID NO:1, a human gene high expression promoter selected from CMV(cytomegalovirus), RSV(Rous sarcoma virus) and beta-actin and loxP gene, wherein E1 and E3 genes required for adenovirus replication are removed from the shuttle vector. A replication-incompetent adenovirus Ad-stTRAIL(KCCM 10743P) produces soluble and secretable homotrimer. An anticancer agent comprises the replication-incompetent adenovirus.