-3 Glutamate decarboxylase gene from lactic acid bacteria of Lactobacillus brevis OPK-3

摘要

The present invention is isolated from Lactobacillus Brevis OPK-3 lactic acid bacteria (KFCC 11330) relates to a method of cloning a glutamate-dicarboxy cyclase gene and these genes. ; cloning methods inclusive of other mate dicarboxy cyclase of the present invention, in the cloning methods of the prior-dicarboxy glutamate cyclase, ; (1) dicarboxyphenoxy glutamate cyclase (GAD) Obtained from the Lactococcus Rock Athletics GAD (Lactococuss lactis GAD) sequence (sequence) of sequence homology with Lactobacillus Planta rum or GAD (Lactobacillus plantarum GAD) in ( homology) to obtain a PCR amplified DNA fragment of GAD by 70% or more for each of the primers, ; (2) a DNA fragment of GAD connected to the cloning vector (ligation) and the step of transforming, ; (3) is connected to a DNA fragment of GAD transformed into the expression vector comprises the step of transforming.