DNA marker Application of breed-specific DNA markers for the use of identifying major pure pig breeds maintaining in Korea

摘要

invention Korea in breeding pigs and possibly to distinguish five varieties to be written, pigs five varieties of Large White, Landrace, relates to a method for determining the Duroc breed. ; To this end, the present invention, currently known KIT and the closely related and seek in the pig where MC1R , and was used as the start codon mutations in the 11-bp duplication in the D-loop region and ND2 genes exhibit species-specific phenomenon on mitrochondria DNA. To determine the variation between varieties to take advantage of the KIT gene splicing area exon17 species were classified as white colored species. Duroc paper from the colored species were classified using the (N121D) of the MC1R gene mutations. But do not be unusual color variation found between the species is more than Duroc classification was not possible. 11-bp duplication events and the D-loop region ND2 White by mutation of the start codon species, Landrace (11-bP weight clothing ATT) species and Large White (11bp duplication and ATA) to classify the species could. Through the methods established by the present invention conclusively determine Landrace, Large White and Duroc purebred identification of species was demonstrated perfectly possible.