METHOD OF PREPARATION OF NEOHESPERIDIN DIHYDROCHALCONE FROM CITRUS

摘要

A method for preparing neohesperidin dihydrochalcone is provided to simply and economically obtain the neohesperidin dihydrochalcone as a high sensitive sweetening agent from citrus pericarp which is a waste material of the citrus. A method for preparing neohesperidin dihydrochalcone from citrus comprises the steps of: (a) biotransforming at least one of hesperetin and hesperetin-7-O-glucoside using at least one microorganism selected from the group consisting of Streptococcus mutans, Rhizobium meliloti and Streptococcus pneumoniae into neohesperidin; and (b) hydrogenating the neohesperidin under the pH of 9-10 to convert the neohesperidin into dihydrochalcone. Before the step(a), the hesperidin extracted from the citrus is treated with at least one enzyme selected from the group consisting of acid, cellulase, pectinase, and amylase or at least one microorganism selected from the group consisting of Aspergillus sp., Bacillus sp., Saccharomyces sp. and Penicillium sp. to prepare hesperetin or hesperetin-7-O-glucoside.