Object e.g. fundus of eye, region`s fluorescence and/or reflection spectra detecting method, involves forming difference signal from accumulated signals for spectral evaluation and presentation of fluorescence and/or reflection spectra

摘要

The method involves selecting a region (2) from an object (1), and confocally reproducing spots in field screens (7, 13) within the selected region from the object. Radiation striking in the field screens is spectrally divided and detected. Local fluorescence and/or reflection signal and external light are accumulated for forming respective signals. A difference signal is formed from the accumulated signals for spectral evaluation and presentation of absolute fluorescence and/or reflection spectra free from an influence of the external light. An independent claim is also included for an arrangement for detecting fluorescence and/or reflection spectra of a selected region of an object e.g. fundus of eye, influenced by an external light.