enon reductase gene and microbial production of levodion

摘要

Disclosed is an isolated DNA comprising a nucleotide sequence coding for an enzyme having enone reductase activity wherein the enzyme is characterized by the following physico-chemical properties: (a) molecular mass: 61,300±5,000 Da (estimated using gel filtration, consisting of one subunit); (b) co-factor: NADPH and NADH; (c) substrate specificity: active on alpha,beta-unsaturated ketons; (d) optimum temperature: 55-60° C. at pH 7.4; and (e) optimum pH: pH 4.5-8.5.