METHOD FOR PRODUCING SAMPLE NUCLEIC ACID FOR ANALYZING NUCLEIC ACID MODIFICATION AND METHOD FOR DETECTING NUCLEIC ACID MODIFICATION USING THE SAME SAMPLE NUCLEIC ACID

摘要

PPROBLEM TO BE SOLVED: To provide a method for analyzing nucleic acid modification by which the analysis can be carried out with smaller amounts of samples than conventional methods and to further provide a method for analyzing a specific site of the genome without requiring complicated operation. PSOLUTION: The method for producing the sample nucleic acids for analyzing nucleic acid modification is provided. The method comprises preparing for a nucleic acid to be the object of detection, dividing the prepared nucleic acid into two groups of a first nucleic acid group and a second nucleic acid group, demodifying the nucleic acid of the first nucleic acid group, carrying out bisulfite modification of the first nucleic acid group and the second nucleic acid group, respectively, performing random amplification of the nucleic acid of each of the groups obtained by the bisulfite modification with a random primer set under amplifiable conditions and providing an amplification product from the first nucleic acid group and an amplification product from the second nucleic acid group as the sample nucleic acids for the genomic analysis. PCOPYRIGHT: (C)2008,JPO&INPIT