THIOFLAVIN T METHOD FOR DETECTION OF AMYLOID POLYPEPTIDE FIBRIL AGGREGATION

摘要

The present invention provides a method for determining the stoichiometric ratio of the amyloid protein and the base which will result in a linear relationship between the amount of amyloid protein and the Thioflavin T fluorescence. Suitable stoichiometric ratios of the amyloid protein to base (such as 10 mM NaOH) of 1 μg:1 μl to 1 μg:1.25 μl can then be used in a Thioflavin T assay to identify potential agents which can inhibit or reduce amyloid protein fibril aggregation.