Disclosed is a method for quantifying analyte molecules in a sample comprising providing a substrate containing two or more reference re- gions containing different immobilised reference first binding partners and one or more analyte regions containing immobilised analyte first binding partner(s); applying a mixture containing reference second bind- ing partners and a sample suspected of containing analyte first or second binding partner(s), in which mixture the concentrations of the reference second binding partners are known; measuring the binding of the bind- ing partner(s) in the mixture to the corresponding immobilised analyte partner(s); and quantifying the concentration of analyte binding part- ner(s) in the sample, where the quantification is performed by correlat- ing the measurement of the binding of the analyte partners to the meas- urement of the binding of the reference binding partners. The method is useful for quantifying microcystin LR.
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