PEPPER CAPMEI1 GENE CODING A PECTIN METHYLESTERASE INHIBITOR PROTEIN AND SCREENING METHOD OF PLANT DISEASE AND ENVIRONMENTAL STRESS RESISTANCE

摘要

A red pepper gene CaPMEI1 encoding a pectin methylesterase inhibitor protein is provided to screen resistance against plant disease and environmental stress rapidly, so that the gene is useful for development of transgenic plants having enhanced resistance against plant disease and environmental stress. A red pepper gene CaPMEI1 encoding a pectin methylesterase inhibitor protein having the amino acid sequence of SEQ ID NO:2 is isolated from Capsicum annuum L. cv. Hanbyul and has the nucleotide sequence of SEQ ID NO:1. A recombinant vector pET32a::CaPMEI1 or pBIN35S::CaPMEI1::GFP contains the red pepper gene CaPMEI1. A method for screening the resistance against plant disease and environmental stress comprises the steps of: isolating RNA from a red pepper plant treated by pathogenic bacterium, chemical or environment stress; subjecting the isolated RNA to electrophoresis and transferring into nylon film(Hybond N+); reacting the nylon film with the CaPMEI1 gene probe labeled with 32P; and confirming discriminative expression of the CaPMEI1 gene.