A plant transformation vector is provided to express an active type plasminogen activator protein by introducing the same into a plant with excellent expression efficiency, thereby being used for developing an effective fibrinolytic enzyme due to very cheap manufacturing cost. A plant transformation vector is characterized in that a TEV(tobacco etch virus) reader sequence having a sequence of SEQ ID : NO. 1, a signal sequence of a glucose-regulated endoplasmic reticular protein derived from Medicago sativa having a sequence of SEQ ID : NO. 2 and a tissue-type plasminogen activator C gene sequence having a sequence of SEQ ID : NO. 3 are linked in sequence. Transgenic bacteria are transformed by the vector, wherein the bacteria are Agrobacterium tumefaciens. A method for preparing a transgenic plant expressing a tissue type plasminogen activator protein or producing the plasminogen activator protein comprises a step of introducing the vector into a plant cell.
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机译:通过将植物转化载体以优异的表达效率引入植物中,提供了表达活性型纤溶酶原激活剂蛋白质的植物转化载体,由于制造成本非常低,因此被用于开发有效的纤溶酶。植物转化载体的特征在于具有序列SEQ ID NO:1的TEV(烟草蚀刻病毒)阅读器序列。参照图1,得自苜蓿的苜蓿的葡萄糖调节的内质网状蛋白的信号序列具有SEQ ID NO:1的序列。 2和组织型纤溶酶原激活物C基因序列具有SEQ ID NO:1的序列。 3个按顺序链接。载体转化了转基因细菌,其中所述细菌是根癌土壤杆菌。一种制备表达组织型纤溶酶原激活物蛋白的转基因植物或生产该纤溶酶原激活物蛋白的方法,包括将载体引入植物细胞的步骤。
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