Vector comprising tissue-type plasminogen activator gene, hairy root transformed thereby and method for preparing tissue-type plasminogen activator using the same

摘要

A recombinant vector, a cell transformed by the vector, a transformed plant cell, and a method for preparing the transformed hairy root are provided to produce the tissue-type plasminogen activator protein massively with a low cost. A recombinant vector comprises the polynucleotide which codes a promoter, a TEV (tobacco etch viru) leader sequence connected with the primer, the glucose-regulated endoplasmic reticular protein derived from alfalfa, and the tissue-type plasminogen activator protein. Preferably the TEV leader sequence has the base sequence represented by the sequence number 1.