METHOD FOR SYNTHESIZING CELL-FREE PROTEIN USING ADENOSINE 3',5'-BIPHOSPHATE, AND SYNTHETIC REACTION SOLUTION FOR CELL-FREE PROTEIN

摘要

PROBLEM TO BE SOLVED: To provide a method for synthesizing a cell-free protein by simply carrying out suppression of mRNA degradation, and to provide a reaction solution capable of conducting synthesis of the cell-free protein by simply carrying out the suppression of the mRNA degradation.;SOLUTION: The synthetic reaction solution for the cell-free protein comprises at least a living cell-derived extract liquid, a potassium salt, a magnesium salt, adenosine triphosphate, guanosine triphosphate, creatine phosphate, creatine kinase, an amino acid, a tRNA, an mRNA, a buffer, and adenosine 3',5'-biphosphate. The method for synthesizing the cell-free protein comprises using the reaction solution.;COPYRIGHT: (C)2009,JPO&INPIT